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Peasy-blunt载体序列

WebNov 5, 2024 · 最近开发了一种叫做同源重组的基因克隆方法， 相比双酶切载体构建方法，该方法的构建过程有些不同。. 首先，靶基因片段扩增引物有别于常规引物设计；其次，载体切割过程中只需要进行单酶切；第三，载体和目的基因片段的连接是基于同源重组而不是粘性 ... WebManual Vector Information MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white …

pEASY®-Blunt E2 Expression Kit

WebApr 13, 2024 · For probe labelling, specific 500 bp sequences of target genes were amplified from the complementary DNA (cDNA) of Wm82 with primers (Supplementary Data 9) and cloned to pEASY-Blunt cloning vector ... WebQuantity：. pEASY® - Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion. •5 minutes fast ligation of Pfu-amplified PCR products. •Ampicillin resistance gene for selection. medal of honor process of nomination https://jd-equipment.com

pEasyBlunt为什么载体 - 百度知道

Web产品详情介绍. pEASY®-Blunt Simple Cloning Vector消除了pEASY®-Blunt Cloning Vector上的多克隆位点，克隆后的PCR产物无法使用pEASY®-Blunt Cloning Vector上多克隆位点的限制性内切酶切下。. 包含LacZ基因，在含有IPTG和X-gal的平板培养基上，可进行蓝白斑筛选， 适用于平端克隆。. WebpEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 … WebAug 7, 2013 · 产品说明pEASY-T1CloningKits适用于TA克隆.-快速克隆基因，仅需要5分钟反应时间。. 对照插入片段克隆效率可达95%以上。. 包含LacZ基因，在含有IPTG，X-gal的平 … medal of honor points navy

pEASY-Blunt clonning vector 载体说明书WORD版 - 百度文库

Category:pEASY-Blunt3 (linearized) Sequence and Map - SnapGene

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Peasy-blunt载体序列

pEASY® -Blunt3 Cloning Kit - TransGen Biotech

WebOct 15, 2009 · 最近在做原核表达载体构建，我的目的片段大小为3.5kb和4.5kb，用的表达载体是全式金的pEASY-Blunt E2，感受态是E.coli DH5a，链接表达载体的体系是pcr产物3ul，ddH2O,1ul，pEASY-Blunt E2加1ul，总共5ul，室温30min，然后就转化感受态细胞加50ul，冰置30min，42℃热激90s，冰置2min ... WebOct 22, 2016 · pEASY-Blunt clonning vector 载体说明书WORD版.doc 22页. pEASY-Blunt clonning vector 载体说明书WORD版.doc. 22页. 内容提供方 ： me54545. 大小 ： 419 KB. 字数 ： 约2.76万字. 发布时间 ： 2016-10-22发布于河南.

Peasy-blunt载体序列

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WebDec 23, 2024 · 质粒的接合转移 ：是细菌遗传物质转移的一个重要方式。. 在质粒转移过程中，供体菌和受体菌通过结合作用紧密接触，质粒从供体细胞向受体转移，同时进行质粒复制。. 按能否自主转移，可以将天然存在的质粒分为转移型质粒和非转移型质粒两大类。. 这里要 ... WebMay 31, 2024 · 分别利用T7引物与M13引物对pEASY质粒和两个pTOPO质粒所连接的片段进行PCR验证，产物进行琼脂糖凝胶电泳后如图1-B所示，pTOPO-ppPig、pTOPO-kpPig、pEASY-Pig重组质粒所连片段（泳道1、2、3），3个泳道的条带均在大于15 000 bp处有明显条带，与预期相符。

http://www.zdsbio.com/index/shopping/details/id/142.html WebAug 3, 2016 · pEASY- T1 Cloning Kits在3’ 端提供一个悬垂的T 碱基，这样的结构使PCR片段可以高效的和载体进行连接。. 牛痘拓扑异构酶I型可以与DNA双链 的特定位置结合并切割特异位点5’- (C/T)CCTT-3’, 与3‘T的磷酸基团性成共价键，并切断其中 一条DNA链，使DNA解链。. …

Web产品详情介绍. pEASY®-Blunt Cloning Vector包含LacZ基因，在含有IPTG和X-gal的平板培养基上，可进行蓝白斑筛选，适用于平端克隆。. WebFigure Legend Snippet: Kinetic properties of recombinant PiGSTd1. (A) Purification of recombinant pEasy-Blunt-E1-PiGSTd1. Induced: the crude extracts from the bacterial pellets with 1mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. 1–4: samples eluted with binding buffers containing 50, 100, 150, and 200mM imidazole, respectively.

WebpEASY-Blunt clonning vector 载体说明书WORD版. LacZfragment: bases 1-548. M13 reverse priming site: bases 205-221. Multiple cloning site: bases 234-358. T7 promoter/priming …

WebOct 22, 2016 · pEASY-Blunt clonning vector 载体说明书WORD版.doc 22页. pEASY-Blunt clonning vector 载体说明书WORD版.doc. 22页. 内容提供方 ： me54545. 大小 ： 419 KB. … penalty for perjuryWeb一、载体概念. 1. 载体，DNA的搬运工. 载体：在基因工程重组DNA技术中，将DNA片段（目的基因）转移至受体细胞的一种能自我复制的DNA分子。. 2. 载体，基因密码承载者. 载体，小人物的大作用. ① 分子较小；. ② 尽可能多限制酶切割位点且单一；. medal of honor ps1 coverWebDownload pEASY-Blunt E1 (linearized).dna file. Download Plasmid Open in SnapGene. SnapGene. SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. Fast accurate construct design for all major molecular cloning techniques; penalty for paying tax bill lateWebpEASY-Blunt3 (linearized) Sequence and Map. Cloning vector for blunt-ended PCR products. The cloned PCR product can be excised with EcoRI or NotI. Cloning vector for blunt-ended … penalty for paying irs lateWebDec 23, 2024 · 质粒的接合转移 ：是细菌遗传物质转移的一个重要方式。. 在质粒转移过程中，供体菌和受体菌通过结合作用紧密接触，质粒从供体细胞向受体转移，同时进行质粒复 … penalty for perjury in marylandWebpEASY-Blunt E1 (linearized) pHY300PLK. pSMART HCAmp. T7 promoter. pEASY-Blunt E2 (linearized) pICOz. pSMART HCAmp (linearized) tac promoter. pEASY-Blunt Simple (linearized) pInvRecA. pSMART HCKan. trc promoter. pEASY-Blunt Zero (linearized) pJAZZ-OC. pSMART HCKan (linearized) phiX174. pEASY-Blunt3 (linearized) medal of honor rag playWeb本发明提供一种检测新型鸭呼肠孤病毒感染抗体的间接ELISA试剂盒，属于生物技术领域的免疫学检测方法。所述新型鸭呼肠孤病毒非结构蛋白p18的序列如SEQ ID No：2所示。本发明检测新型鸭呼肠孤病毒感染抗体的间接ELISA试剂盒，能够区分抗体是源于病毒感染还是灭活疫苗免疫，从而可以移除新型鸭呼 ... medal of honor ps3 game