http://www.caclp.com/article/?detail_5304.html WebMay 10, 2016 · The study diagnostic kit in this study was coded ODK-1201. It is based on a HawkZ05 Fast One-Step RT-qPCR Kit (Roche, Indianapolis, IN, USA). In this kit, reverse transcription and quantitative PCR are performed in one tube. The analysis was carried out on an ABI™ 7500 Fast Dx system (Applied Biosystems, Foster City, California).
Odk-1201, One-Step RT-qPCR Major - ScienceDirect
WebHawkZ05 Fast Polymerase is marketed as a fast RT-qPCR assay. The manufacturer recommends performing the RT step for 2 to 5 minutes (4). It was tested how reaction conditions involving higher levels of primer and enzyme would affect the RT-qPCR assay. Surprisingly, very similar Cqs were measured when the RT time was 5 min, 2 min, 30 sec., WebMay 10, 2016 · The study diagnostic kit in this study was coded ODK-1201. It is based on a HawkZ05 Fast One-Step RT-qPCR Kit (Roche, Indianapolis, IN, USA). In this kit, … fresh minecraft memes
WO2024150442A1 - Rapid reverse transcription quantitative …
WebHawkZ05 Fast Polymerase is marketed as a fast RT-qPCR assay. The manufacturer recommends performing the RT step for 2 to 5 minutes (4). It was tested how reaction … WebDec 6, 2014 · Methods: An ODK-1201 kit (Otsuka Pharmaceutical Co. Japan) is based on a HawkZ05 Fast One-Step RT-PCR Kit (Roche, Indianapolis, IN, USA). In this kit, reverse transcription and quantitative PCR ... WebMar 15, 2013 · Reactions were performed using the HawkZ05™ Fast One-Step RT-PCR Master mix in a single-tube format with unmodified (U) For and Rev PCR primers or blocked-cleavable (B) For and Rev rhPCR primers. The reverse transcription (RT) reaction was done using 10 ng of HeLa cell RNA and proceeded in a stepwise fashion with incubations of 5 … freshminds nz